![]() ![]() In summary, our data convincingly argue that egg white can be used as a suitable alternative model for 3-D cell culture studies. We also cultured different established cell lines, oncogene-transformed MCF10A, and mouse mammary epithelial cells in egg white and BM, and observed similar morphology. There was no significant difference in MCF10A proliferation and acinar size between egg white and BM. Specifically, we show that growth of immortalized human breast epithelial cells (MCF10A) in egg white–based medium results in formation of acini with hollow lumens, apoptotic clearance of the cells in the lumen, and apicobasal polarization comparable to what has been described using established 3-D culture media such as reconstituted basement membrane preparations (BM). Here we show a simple system based on avian egg white that supports growth of cells in 3-D, at a significantly decreased cost. ![]() Although three dimensional (3-D) cell culture systems have numerous advantages over traditional monolayer culture, the currently available 3-D cell culture media are cost-prohibitive for regular use by the majority of research laboratories. ![]()
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